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Minelute pcr purification kit

Minelute pcr purification kit

Qiagen qiacube demonstration by new life scientific

PMID 11850822 explains how LNCaP prostate cancer cells were cultured. Standard prostate epithelial cells (PrEC) were cultured in prostate epithelial growth medium (PrEGM, catalogue no. CC-3166; Cambrex Bio Science) according to the manufacturer’s instructions, as defined in PMID 20173741. At passage 73, LNCaP cells were used, and at passage 6, PrEC cells were used.
Version 1.2 of an internally built pipeline, http://github.com/astatham/Bisulfite tools, was used to match bisulfite reads to the human genome. Trimgalore (version 0.2.8, http://www.bioinformatics.babraham.ac.uk/projects/trim galore/) was used in paired-end mode with default parameters to delete adaptor sequences and low quality bases. The parameters “-p 4 –bowtie2 –X 1000 –unmapped –ambiguous –gzip –bam” were then used in Bismark v0.8.3 [PMID: 21493656] to match reads to hg19. Picard v1.91 (http://broadinstitute.github.io/picard) was used to delete PCR duplicates. Bismark methylation extractor with the parameters “-p –no overlap –ignore r2 4 –comprehensive –merge non CpG –bedgraph –counts –report –gzip –buffer size 20G” was used to create count tables of the number of methylated and unmethylated bases sequenced at each CpG site in the genome. Supplementary files format and content: hg19Genome build: hg19Supplementary files format and content: tsv file containing methylated (C) and coverage (cov, methylated Cs + unmethylated Ts) counts at each CpG site assayed

Protocolo qiaamp minelute virus spin en qiacube

Purification of PCR items up to 5 g In low elution concentrations, 70 bp to 4 kb Contents of the kit 50 MinElute Spin Columns from Qiagen MinElute PCR Purification Kit Binding Capacity: 5g Elution Volume Tube Format of 10L Silica Science and Technology 70 beats per minute to 4 kilobytes Size of the Fragment DNA Sample Processing by Hand Procedural Speed and Handling Ease Recoveries that are highly repeatable Purification of PCR Products up to 5g in Low Elution Volumes 50 MinElute Spin Columns are included. Buffers are a type of storage device. Tubes with a capacity of 2mL Advantages Volumes of elution are extremely small. Procedure is fast and simple to handle. High recoveries that are repeatable For easy sample analysis, use a gel loading dye.
Snippet from the article: GelRed nucleic acid gel stain (Biotium, Hayward, USA) was used to visualize PCR products electrophoresed on 2.0 percent agarose gels. A MinElute PCR purification kit (Qiagen) was used to purify the PCR product according to the manufacturer’s protocol. Big Dye Terminator v3.1 Cycle Sequencing was used to directly sequence amplicons in both directions. Ready reaction kit (Applied Biosystems, Foster City, USA) in an Applied Biosystems 3100 Automated DNA Sequencer on the RPT01A/IOC- Fiocruz sequencing network, as recommended by the supplier. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation, according to the study

Automated nucleic acid purification from diverse sample types

For silica-membrane-based purification of PCR products 70 bp – 4 kb in size, the MinElute PCR Purification Kit includes spin columns, buffers, and collection tubes. The spin columns are engineered to produce high yields of highly concentrated DNA in small volumes (as little as 10 l). The optimal pH for DNA binding to the spin column can be easily determined with the help of an optional pH indicator. On the QIAcube Link, the procedure can be fully automated.
Primers, nucleotides, enzymes, mineral oil, salts, and other impurities are removed from DNA samples using the MinElute PCR Purification process (see figure “Efficient primer removal”). Spin columns are included in the MinElute PCR Purification Package for PCR product cleanup. Large concentrations of DNA fragment (70 bp – 4 kb) can be achieved easily using a microcentrifuge or vacuum manifold. (Use the QIAquick PCR Purification Kit to purify DNA fragments larger than 4 kb.)
A silica membrane assembly is included in the MinElute PCR Purification Kit for binding DNA in high-salt buffer and elution with low-salt buffer or water. The issues and inconveniences associated with loose resins and slurries are removed with silica-membrane technology. Dye for gel loading Gel loading dye is provided to allow faster and more convenient sample processing and analysis. GelPilot Loading Dye includes three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to help in agarose gel run time optimization and prevent smaller DNA fragments from migrating too far (see figure “GelPilot Loading Dye”).

Minelute 2009 : 06 : qiacube overview

For silica-membrane-based purification of PCR products 70 bp – 4 kb in size, the MinElute PCR Purification Kit includes spin columns, buffers, and collection tubes. The spin columns are engineered to produce high yields of highly concentrated DNA in small volumes (as little as 10 l). The optimal pH for DNA binding to the spin column can be easily determined with the help of an optional pH indicator. On the QIAcube Link, the procedure can be fully automated.
GelPilot Loading Dye.|MinElute membrane.|pH Indicator Dye.|MinElute procedure.|Efficient primer removal.|GelPilot Loading Dye.|MinElute membrane.|pH Indicator Dye.
Options for treating spin columns Spin column handling options — D.|Spin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|A.|
Primers, nucleotides, enzymes, mineral oil, salts, and other impurities are removed from DNA samples using the MinElute PCR Purification process (see figure “Efficient primer removal”). Spin columns are included in the MinElute PCR Purification Package for PCR product cleanup. Large concentrations of DNA fragment (70 bp – 4 kb) can be achieved easily using a microcentrifuge or vacuum manifold. (Use the QIAquick PCR Purification Kit to purify DNA fragments larger than 4 kb.)