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Invitrogen 1 kb plus dna ladder

Invitrogen 1 kb plus dna ladder

How to prepare and load a standard dna ladder

The Invitrogen 1 Kb Plus DNA Ladder is used to size and estimate quantify double-stranded DNA with lengths ranging from 100 to 15,000 base pairs. The 1 Kb Plus DNA Ladder is made up of 18 chromatography-purified DNA fragments with a 1,500 bp reference band for fast orientation. On 0.8–1% agarose gels, the 1 Kb DNA Ladder is suitable for separation. 1 Kb Plus DNA Ladder Highlights: • Sharp, transparent bands—chromatography filtered fragments for repeatable and dependable results • Practical—includes 10X BlueJuice Gel Loading Buffer for monitoring sample DNA migration. • Accurate—each band contains an exact amount of DNA. Usage of the product After staining with ethidium bromide or SYBR Secure, the double-stranded DNA ladder can be seen on 0.8–1% agarose gels. For a clear view of each band, the ladder is constructed with a uniform strength of DNA bands. The approximate quantification of DNA samples is possible since each band contains an exact amount of DNA. T4 polynucleotide kinase or T4 DNA polymerase may be used to radiolabour this ladder.

Plasmid dna transfection protocol

The 1 kb Plus DNA Ladder is an important method for measuring the molecular weight of linear, double-stranded DNA fragments. The 1 kb Plus DNA ladder is made up of 20 highly purified double-stranded DNA bands ranging in size from 100 to 12,000 base pairs. This ladder has 12 uniformly spaced bands ranging from 1 to 12 kb, a 1,650 bp fast orientation band that forms a distinct doublet with the 2 kb band, and seven round bands below 1 kb. The E-Gel® pre-cast agarose gels, as well as agarose gels cast in your lab, are compatible with the 1 kb Plus DNA ladder.

Dial up your cleanup: increase efficiency with genejet

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Electrophoresis: how to read results

The Invitrogen 1 Kb Plus DNA Ladder is used to scale and approximate measure double-stranded DNA in the range of 100 to 15 000 base pairs. The 18 individual chromatography purified DNA fragments in the 1 Kb Plus DNA Ladder have a reference band of 1 500 bp for easy orientation. On 0 8 1 agarose gels, the 1 Kb DNA Ladder is suitable for separation. • Sharp clear bands chromatography purified fragments for consistent and accurate performance • Convenient 10X BlueJuice Gel Loading Buffer for monitoring sample DNA migration • Precise an exact amount of DNA in each band Usage of the product After staining with ethidium bromide or SYBR Secure, the double-stranded DNA ladder can be seen on 0 8 1 agarose gels. For a clear view of each band, the ladder is constructed with a uniform strength of DNA bands. The approximate quantification of DNA samples is possible since each band contains an exact amount of DNA. T4 polynucleotide kinase or T4 DNA polymerase may be used to radiolabour this ladder.