How is total magnification calculated
How to calculate total magnification on a compound
A compound microscope is a microscope that enlarges the image of a sample by using multiple lenses. A compound microscope is typically used to display samples at high magnification (40-1000x), which is accomplished by combining the effects of two lenses: the ocular lens (in the eyepiece) and the objective lenses (close to the sample).
To modify the magnification, compound microscopes typically have interchangeable objective lenses of various magnifications (e.g. 4x, 10x, 40x, and 60x) mounted on a turret. A condenser lens and an iris diaphragm are also included in these microscopes, which are critical for controlling how light hits the sample.
The stereo- or dissecting microscope is an optical microscope variant equipped for low magnification (2-100x) observation using incident light illumination (light reflected off the sample surface is detected by the user), but it can be combined with transmitted light in some instruments. To provide slightly different viewing angles to the left and right eyes, it uses two distinct optical paths with two targets and two eyepieces. As a result, the sample can be visualized in three dimensions.
How to calculate magnification
The overall magnification of the microscope is determined by multiplying the objective’s magnifying power by the eyepiece’s magnification and, where applicable, by intermediate magnifications.
The lateral magnification is always an observable value, while the magnification always refers to the impression of the eye. The magnification is 1x when an object is viewed with the eye from a distance of 250 mm. When the distance between the target and the observer is 500 mm, the size of the object seen is halved, and the magnification is decreased to 0.5x. A key aspect is the viewing angle from which something is viewed. When looking at a microscope, the viewing angle through which the object is seen is improved by the factor arising from the total magnification equation above. This does not imply that the eye is able to picture the entire viewing angle! The field of view limits the viewing angle.
If an image is formed that will be measured with a scale, the term lateral magnification is often used. For example, a picture taken with a microscope camera is used to determine the size of an object detail in a specimen. The photo’s detail is calculated using a scale for this. The original scale is obtained by dividing this length by the total magnification. Example: 10 mm object on paper picture, taken with objective 40x, phot eyepiece 10x, camera magnification 0.25x, and magnified 4x from negative.
Calculating magnification on a compound microscope
Most compound microscopes come with objective lenses, which are interchangeable lenses. The most common magnification powers for objective lenses are 4x, 10x, 40x, and 100x, which are also known as scanning, low power, high power, and (typically) oil immersion objectives. Let’s take a closer look at the various magnifications of objective lenses and when they may be useful.
The magnification power of a scanning objective lens is the lowest of all objective lenses. A 4x scanning objective lens, when combined with the magnification capacity of a 10x eyepiece lens, provides a cumulative magnification of 40x. The word “scanning” objective lens refers to the fact that they provide viewers with only enough magnification to get a clear overview of the slide, basically a “scan.” Specialty Goals, which have even lower strength, are discussed below.
The low power objective lens magnifies glass slide samples more than the scanning objective lens, and it is one of the most useful lenses for viewing and analyzing them. A low power objective lens paired with a 10x eyepiece lens gives you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes, resulting in a total magnification of 100x.
How to calculate the total magnification of a compound
When viewing a microscopy image on a wide screen, a common question is, “What is the total magnification on my monitor?” We must know the answers to the following four questions in order to calculate the correct answer to this question.
The magnification of the microscope objective lens is printed on the side of the lens, as seen in the image to the left (1). The objective lens value is written on the zoom knob or the objective turret that is rotated to adjust the microscope magnification when using a stereo microscope.
The c-mount adapter (2) connects the microscope and the microscope camera. A number will be written on the side of the c-mount adapter. The c-mount adapter in the picture above has a built-in 0.5x lens.
The diagonal measurement of the display in mm is the next number we need to work out (3). The measurement of a monitor is usually given in inches. We will simply convert the inches to millimeters. To translate this number to mm, multiply it by 25.4 if you’re using a 19″ display. Diagonal display measurement: 19″ x 25.4 = 482.6mm