Cell culture surface area
Surface area to volume ratio of cells | cell structure and
Community of human ARPE-19, A549, and Malme-3M cells. ARPE-19 cells were grown in DMEM/F12 Ham’s medium supplemented with 10% v/v FBS, 2 mM L-glutamine, and a penicillin-streptomycin solution containing 100 IU/ml penicillin and 100 g/ml streptomycin. The A549 and Malme-3M cells, on the other hand, were cultured in DMEM with 10% v/v FBS, 2 mM L-glutamine, and a 100 IU/ml penicillin-100 g/ml streptomycin solution. Cells were grown in T-75 tissue culture flasks and held in a humidified-atmosphere incubator at 37°C and 5% CO2 for routine maintenance. The cells were detached using a standardised trypsinisation method with 0.25 percent v/v trypsin-EDTA in PBS when cell confluency reached 85 percent (approximately every 2–3 days).
Surface area calculation for cell development.
Within the required experimental set-up, care must be taken to ensure that both fixed surface area (FSA) and fixed volume (FV) are preserved during culture. As a result, the following calculations show that adding 100 l of cell suspension to a flat well plate gives the cultured cells a surface area of 94.6 mm2. The volumes of growth medium needed to achieve the same growth surface area for cells on round and v-bottom plates were calculated using this information: 117 l for round well plates and 123 l for v-shaped well plates. It should be remembered that the corona discharge process prepares and treats the entire plate and lid (i.e. the total exposed surface area) for all Corning well plates (information provided by Corning Life Sciences, UK).
The impact of sparging on cell culture in bioreactors – two
The availability of both growth surface and medium components will restrict the growth of untransformed fibroblasts in culture, according to studies. Experiments with cells grown on coverslips, where the only variable was the available growth surface, show that when the medium to cell ratio is high, surface area is the primary limiting factor. However, at low medium to cell ratios, medium components are the primary limiting factor in cell growth. When the culture medium is modified regularly, the amount of cells per culture is almost directly proportional to the available surface area.
Falcon® cell culture multi-flask | multiply your cell growth
The total cell surface area can increase when a cell divides into two daughter cells. Exocytosis of intracellular vesicles and unfolding of small surface membrane reservoirs such as microvilli or wrinkles are two models for membrane supply to sustain cell division. We calculated the total cell surface area of dividing Dictyostelium cells, which had been flattened by an agar overlay, which removed the difficulty of unfolding surface membrane reservoirs. Unfolding of surface membrane reservoirs was not needed for cell division because the cells divided normally under the agar overlay. The total cell surface area decreased marginally from interphase to metaphase under the agar overlay, then increased by about 20% during cytokinesis. In the early mitotic stages, both endocytosis and exocytosis were suppressed, but they recovered during cytokinesis. The differences in cell surface area may be due to an imbalance of endocytosis and exocytosis. Clathrin-dependent endocytosis was also significantly reduced during cytokinesis, but it did not contribute significantly to the regulation of cell surface area, contrary to previous reports in cultured animal cells. Furrowing was necessary for cell membrane expansion during cytokinesis, and vice versa.
Spl cell culture slide & hybridwell
TPP tissue culture dishes are the best choice for adherent cell culture in research and industry because of their revolutionary features and superior cell growth properties. To prevent cell attachment on the edge, these optically clear, high-grade polystyrene tissue culture dishes are tissue culture treated only on the base of the bowl, with a non-treated ring surrounding the rising surface. A serrated ring around the bottom culture dish makes it secure and convenient to pick up and treat the dishes, while also reducing the chance of dishes falling due to lid/base disassembly.
The tissue culture dishes can be safely and securely stacked using a special stacking ring with spaces for a numeric scale (12, 3, 6, 9) set on the periphery. The numeric scale helps in identifying areas of inspection, while the spaces produced in the stacking ring help to prevent condensation between stacked dishes and tissue culture dishes from sticking together.
The top dish wall (yellow) and the bottom dish wall (frosted) have two marking areas that serve as a guide for top/bottom alignment and orientation. Six special stops are built into the lid to allow for efficient gas exchange during incubation.