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384 well plate volume

384 well plate volume

Viaflo 96 / viaflo 384 – electronic 24-, 96- and 384

Advanced PCR (polymerase chain reaction) techniques are needed by advances in molecular biology. With the rise of high-throughput PCR and shorter cycle times, reaction vessels that produce reliable, dependable results are in high demand.
We conducted individual experiments to compare Corning polyolefin plates with comparable PCR plates from two other manufacturers (A + B) in order to determine the benefits of Corning’s 384 well PCR plates for high throughput applications. The plates were examined for cross contamination between wells, fluid loss due to evaporation and wicking, and amplification signal accuracy and efficiency across the plate’s surface. The reaction volumes ranged from 5 to 25 liters. For high-throughput PCR applications, this Technical Note serves as a benchmark for evaluating reaction conditions and selecting consumables.
This analysis contrasted comparable 384 well PCR plates (catalog no. 6502), Aluminum Sealing Tapes-384 (catalog no. 6569), and Reusable Sealing Mats (catalog no. 3699) from two manufacturers (A + B).

Taqman assay formats explained – ask taqman #29

It is difficult to ignore the fact that the workload in laboratories is continuously rising. Working with 96-well plates may have been progressive 25 years ago, but a rising number of labs are now transitioning to the 384-well plate format. Super, you now have four 96-well plates on a single plate – but with smaller wells. But what is the benefit? At first glance, this device seems to have just drawbacks – it’s time to take a closer look.
Expensive reagents like those used in molecular biology can be saved, lowering the cost per study. Naturally, this is only possible if the reaction or analysis can be made smaller. Is it possible to manually fill a 384-well plate in under a minute?
Another benefit is that you save time. Is it possible to manually fill a 384-well plate in under a minute? With 8/12-channel pipettes, 384-well plates can be loaded. However, each second well can only be filled once.
On the one hand, it’s prone to errors (“oh, my centrifuge is ready… which well was the next to be filled?”). It is, however, exhausting and time-consuming. It’s a waste of money to convert the plates to 384-well format and fill them with 8/12-channel pipettes.

Corning 4514 384 well plate, low volume, black, nbs, u

The detection limit in SmallVolumeTM microplates using classical microplate readers can be significantly improved by optimizing the Z-axis according to the liquid height to be measured in the wells, and a detection limit comparable to that of the 1536 well microplate can be achieved.
The detection limit in SmallVolumeTM microplates using classical microplate readers can be significantly improved by optimizing the Z-axis according to the liquid height to be measured in the wells, and a detection limit comparable to that of the 1536 well microplate can be achieved.

Amplify sample with the viia™ 7 real-time pcr system

Clear solid 384 well plates with low fluorescence are made of pure polystyrene and can be used for ELISA assays as well as animal and plant cell tissue culture studies. The wells’ inner bottoms have a radiused edge that increases washing efficiency. The 384 well plates minimize sample volume while increasing screening quality. The plates meet SBS specifications, and the design ensures that they can perform well in an automated processing plant.
Tissue culture treated plates provide the best, most consistent, and most compatible surface for animal cell attachment and development. This surface was produced using a proprietary vacuum plasma treatment with tightly regulated parameters that ensures the surface’s consistency of behavior. The surface chemistry produces a consistent surface that is both hydrophilic and negative in charge.